The present results thus show that the cis-9, trans-11 + trans-8, cis-10 CLA mixture can deteriorate plasma VLDL cholesterol and triglycerides in hamsters, possibly due to an increased flux of glucose. No differences in body composition were noted between the three groups. The cis-9, trans-11 + trans-8, cis-10 CLA mixture also induced the highest plasma glucose concentrations compared with the two other groups, indicating an impairment of glycemic control. On the other hand, the cis-9, trans-11 + trans-8, cis-10 CLA mixture induced higher plasma VLDL cholesterol and triglycerides than LA and the cis-9, trans-11 + trans-10, cis-12 CLA mixture. The cis-9, trans-11 + trans-8, cis-10 CLA mixture induced similar plasma LDL cholesterol and hepatic lipid concentrations, and coefficient of digestibility as LA, indicating no effect of the trans-8, cis-10 CLA isomer on these lipid parameters. The cis-9, trans-11 + trans-10, cis-12 CLA mixture showed higher LDL cholesterol concentrations than LA and the cis-9, trans-11 + trans-8, cis-10 CLA mixture. The objective of the present study was to compare the effects of cis-9, trans-11 + trans-8, cis-10 conjugated linoleic acid (CLA) mixture to those of cis-9, trans-11 + trans-10, cis-12 CLA mixture and linoleic acid (LA) on lipoprotein profile, hepatic lipids, body composition and digestibility of dietary fat in hamsters ( n = 17) fed diets containing 2% of experimental fat (w/w) for 28 days.